Services

• Microbial culture
  • Large scale bacterial culture for protein expression or cell product
  • Culture of enteric bacterial pathogens or intestinal parasites (Giardia lamblia, E. histolytica)
  • Microbial storage and maintenance of collections
    
• Plasmid preps
  • Maxipreps, for transfection
  • >40Kb plasmids
  • Standard plasmid vectors and reporter genes
    
• Competent cells
  • Chemically competent (CaCl2 competent)
  • Electrocompetent
    
• Protein purification
  • Taq polymerase
  • Shiga toxins and other bacterial virulence proteins
  • Fusion proteins, e.g., maltose binding protein, 6-his, GST
    
• Media preparation
  • Liquid media
  • Agar plates

The Phoenix Lab