Fortin JP, Zhu Y, Choi C, Beinborn M, Nitabach MN, Kopin AS. Membrane-tethered ligands are effective probes for exploring class B1 G protein-coupled receptor function. Proceedings of the National Academy of Sciences, USA 2009; 106(19):8049-8054.
Stanic D, Paratcha G, Ledda F, Herzog H, Kopin AS, Hökfelt T. Peptidergic influences on proliferation, migration, and placement of neural progenitors in the adult mouse forebrain. Proceedings of the National Academy of Sciences, USA 2008;105(9):3610-3615.
Al-Fulaij MA, Ren Y, Beinborn M, Kopin AS. Pharmacological Analysis of Human D1 and D2 Dopamine Receptor Missense Variants. Journal of Molecular Neuroscience 2008;34(3):211-223.
Liu G, Fortin JP, Beinborn M, Kopin AS. Four Missense Mutations in the Ghrelin Receptor Result in Distinct Pharmacological Abnormalities. Journal Pharmacology Experimental Therapeutics 2007;322(3):1036-1043.
Bi S, Chen J, Behles RR, Hyun J, Kopin A, Moran TH. Differential body weight and feeding responses to high fat diet in rats and mice lacking cholecystokinin 1 receptors. American Journal of Physiology - Regulatory, Integrative and Comparative Physiology 2007;293(1):R55-R63.
Al-Fulaij MA, Ren Y, Beinborn M, Kopin A. Identification of Amino Acid Determinants of Dopamine 2 Receptor Synthetic Agonist Function. Journal of Pharmacology and Experimental Therapeutics 2007;321(1):298-307.
Draper I, Kurshan P, McBride EW, Jackson FR, Kopin AS. Locomotor activity is regulated by D2-like receptors in Drosophila: An anatomic and functional analysis. Developmental Neurobiology 2007;67(3):378-393.
Whited KL, Thao D, Lloyd KC, Kopin AS, Raybould HE. Targeted disruption of the murine CCK1 receptor gene reduces intestinal lipid-induced feedback inhibition of gastric function. American Journal of Physiology - Gastrointestinal and Liver Physiology 2006;291(1):G156-G162.
Mukherjee RS, McBride EW, Beinborn M, Dunlap K, Kopin AS. Point mutations in either subunit of the GABAB receptor confer constitutive activity to the heterodimer. Molecular Pharmacology 2006;70(4):1406-1413.
Blow NS, Salomon RN, Garrity K, Reveillaud I, Kopin A, Jackson FR, Watnick PI. Vibrio cholerae infection of drosophilamelanogaster mimics the human disease cholera. PLoS Pathogens 2005;1(1):92-98.
Beinborn M, Worrall CI, McBride EM, Kopin AS. A human glucagon-like peptide-1 receptor polymorphism results in reduced agonist responsiveness. Regulatory Peptides 2005;130(1-2):1-6.
Kopin, A.S., McBride, E.W., Schaffer, K., Beinborn, M. CCK receptor polymorphisms: an illustration of emerging themes in pharmacogenomics. Trends in Pharm. Sciences. 2000;21(9):319-359.
Hearn MG, Ren Y, McBride EW, Reveillaud I, Beinborn M, Kopin AS. A Drosophila dopamine 2-like receptor: Molecular characterization and identification of multiple alternatively spliced variants. Proc Natl Acad Sci USA 2002;99 (22): 14554-14559.
Kopin AS, McBride EW, Chen C, Freidinger RM, Chen D, Zhao C, Beinborn M. Identification of a series of CCK-2 receptor nonpeptide agonists: sensitivity to stereochemistry and a receptor point mutation. Proc. Natl. Acad. Sci.2003;100(9):5525-30.
Beinborn M, Ren Y, Bläker M, Chen C, Kopin AS. Ligand function at constitutively active receptor mutants is affected by two distinct yet interacting mechanisms. Molecular Pharmacology 2004;65(3):753-760.
Wang DQH, Schmitz F, Kopin AS, Carey MC. Targeted Disruption of the Murine Cholecystokinin-1 Receptor Promotes Intestinal Cholesterol Absorption and Susceptibility to Cholesterol Cholelithiasis. Journal of Clinical Investigation 2004;114(4): 521-541.
Alan S. Kopin is a Professor of Medicine, and the director of the Molecular Pharmacology Research Center at Tufts Medical Center. He is also a Professor of Pharmacology and Genetics at the Sackler School, Tufts University School of Medicine. Dr. Kopin received his B.A. in biology from Brown University and M.D. from the University of North Carolina School of Medicine. His research training included fellowships in physiology at Harvard Medical School and in gastroenterology at Tufts Medical Center. Dr. Kopin is currently board certified in internal medicine and in gastroenterology.
The Kopin laboratory has studied the molecular pharmacology of G protein-coupled receptors (GPCRs) for two decades. Special interests include: structure-function relationships, GPCR mediated physiology, polymorphism/ mutation induced alterations in function, and the development of tools for drug discovery. Ongoing studies in the laboratory have clinical relevance in the areas of metabolic disease (i.e. obesity and diabetes), neurologic/locomotor dysfunction (e.g. Parkinson’s disease) and nociception (e.g. acute pain).
Current investigations are aimed at:
understanding the molecular basis for peptide hormone- GPCR interaction and function
exploring how genetic variation in selected receptors influences the onset and/or progression of disease
studying the pharmacological properties and potential medical applications of membrane-tethered ligands, an innovative technology developed in the laboratory to modulate GPCR function, (iv) generating recombinant protein activators of orphan GPCRs
identifying novel compounds which mediate GPCR activity. To accomplish these objectives, the laboratory utilizes molecular biologic tools, cell-based assays, as well as animal models (e.g. genetically altered mice and fruit flies).
Work in the Kopin laboratory is currently supported by grants from the Michael J. Fox Foundation, NIDDK and NIDA.
Molecular Pharmacology Research Center
Molecular Cardiology Research Institute
Tufts Medical Center
800 Washington Street, Box #80
Boston, MA 02111