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Janis Lem, PhD


Overview

Training + Education University of Southern California; California Institute of Technology
Gender Female

Publications + National Presentations

Wen XH, Shen L, Brush RS, Michaud N, Al-Ubaidi MR, Gurevich VV, Hamm HE, Lem J, Dibenedetto E, Anderson RE, Makino CL. Overexpression of rhodopsin alters the structure and photoresponse of rod photoreceptors. Biophysical Journal 2009;96(3):939-950.

Lem J, Rwayitare K. Transgenic strategies for the analysis of photoreceptor function. In: Signal Transduction in the Retina. S. Fliesler and O. Kisselev, 2008;Chapter 7:145-161.

Brill E, Malanson KM, Radu RA, Boukharov, NV, Wang Z, Chung H-Y, Lloyd MB, Bok D, Travis GH, Obin M, Lem J. A novel form of transducin-dependent retinal degeneration: Accelerated retinal degeneration in the absence of rod transducin. Investigative Ophthalmology & Visual Science 2007;48(12):5445-5453.

Rosenzweig DH, Nair KS, Wei J, Wang Q, Garwin G, Saari JC, Chen CK, Smrcka AV, Swaroop A, Lem J, Hurley JB, Slepak VZ. Subunit Dissociation and Diffusion Determine the Subcellular Localization of Rod and Cone Transducins. Journal of Neuroscience 2007;27(20):5484-5494.

Rajala A, Anderson RE, Ma JX, Lem J, Al-Ubaidi MR, Rajala RV. G-protein-coupled receptor rhodopsin regulates the phosphorylation of retinal insulin receptor. Journal of Biological Chemistry 2007;282(13):9865-6873.

Bemelmans AP, Kostic C, Hornfeld D, Jaquet M, Crippa SV, Hauswirth WW, Lem J, Wang Z, Schorderet DE, Munier FL, Wenzel A, Arsenijevic Y. Lentiviral vectors containing a retinal pigment epithelium specific promoter for leber congenital amaurosis gene therapy. Lentiviral gene therapy for LCA. Advances in Experimental Medicine and Biology 2006;572:247-253.

Srinivasan VJ, Ko TH, Wojtkowski M, Carvalho M, Clermont A, Bursell SE, Song QH, Lem J, Duker JS, Schuman JS, Fujimoto JG. Noninvasive volumetric imaging and morphometry of the rodent retina with high-speed, ultrahigh-resolution optical coherence tomography. Investigative Ophthalmology & Visual Science 2006;47(12):5522-5528.

Bemelmans AP, Kostic C, Crippa SV, Hauswirth WW, Lem J, Munier FL, Seeliger MW, Wenzel A, Arsenijevic Y. Lentiviral gene transfer of Rpe65 rescues survival and function of cones in a mouse model of leber congenital amaurosis. PLoS Pathogens 2006;3(10):1892-1903.

Moussaif M, Rubin WW, Kerov V, Reh R, Chen D, Lem J, Chen CK, Hurley JB, Burns ME, Artemyev NO. Phototransduction in a transgenic mouse model of Nougaret night blindness. Journal of Neuroscience 2006;26(25):6863-6872.

Nikonov SS, Kholodenko R, Lem J, Pugh EN Jr. Physiological features of the S- and M-cone photoreceptors of wild-type mice from single-cell recordings. Journal of General Physiology 2006;127(4):359-374.

Maeda T, Lem J, Palczewski K, Haeseleer F. A critical role of CaBP4 in the cone synapse. Investigative Ophthalmology & Visual Science 2005;46(11):4320-4327.

Wang Z, Wen XH, Ablonczy Z, Crouch RK, Makino CL, Lem J. Enhanced shutoff of phototransduction in transgenic mice expressing palmitoylation- deficient rhodopsin. Journal of Biological Chemistry 2005;280(26):24293-24300.

Hao, W., Wenzel, A., Obin, M.S., Chen, C-K., Brill, E., Krasnoperova, N.V., Eversole-Cire, P., Kleyner, Y., Taylor, A., Simon, M.I., Grimm, C., Remé, C.E. and Lem, J. Evidence for Two Apoptotic Pathways in Light-Induced Retinal Degenerations. Nature Genetics 2002;32:254-260.

Calvert, P.D., Krasnoperova, N.V., Lyubarsky, A.L., Isayama, T., Nicolo, M., Kosaras, B., Wong, G., Gannon, K.S., Margolskee, R.F., Sidman, R.L., Pugh, E.N. Jr., Makino, C.L., and Lem, J. Phototransduction in transgenic mice after targeted deletion of the rod transducin a-subunit. PNAS 2000;97:13913-13918.

Lem, J., Flannery, J.G., Li, T., Applebury, M.L., Farber, D.B., and Simon, M.I. Retinal degeneration is rescued in transgenic rd mice by expression of the cGMP phosphodiesterase b-subunit. Proc. Natl. Acad. Sci. 1992;89: 4422-4426.

Lem, J., Krasnoperova, N., Calvert, P., Nicolo, M., Cameron, D., Kosaras, B., Makino, C. and Sidman, R. Morphological, Physiological and Biochemical Changes in Rhodopsin Knockout Mice. Proc. Natl. Acad. Sci. USA 1999;96:736-741.

Biography

Janis Lem is an Investigator in the Molecular Cardiology Research Institute and Eye Center of Tufts Medical Center, and an Associate Professor at Tufts University School of Medicine. She also has an appointment in the Program in Genetics at Tufts University School of Medicine. Dr. Lem received a B.S. in Biology (1979) and a Ph. D. in Microbiology (1987) from the University of Southern California. She completed her postdoctoral training at the California Institute of Technology in 1993, after which she joined the Tufts faculty.

Contact Informatioin

Phone: 617-636-5045

Email: JLem@tuftsmedicalcenter.org

Molecular Cardiology Research Institute
Tufts Medical Center
800 Washington Street, Box #5045
Boston, MA 02111