1999-2003 Fellowship award from the Spanish Ministry of Science and Education
2002 Short-term travel internship award, Spanish Ministry of Science and Education
2003 PhD, cum laude, Department of Molecular Biology, University Autonoma of Madrid, Madrid, Spain
2004-2006 Fulbright award. Post-doctoral Grant at Harvard University , Boston, MA
2004 Alcaide et al, Int Immunol, 2004, 16 (10): 1365. Selected article of the month by the journal International Immunology
2007 Alcaide et al, Jour Exp Med, 2007, 204:431. Selected by the journal Cell, as Leading Edge Research
2008 Alcaide et al, Blood, 2008, 112:2770. Selected by the journal Nature Immunology as Research Highlights
2009-2014, NIH Pathway to Independence award. Brigham and Women’s Hospital and Harvard University,Boston, MA
2013-2014, The Russo Family Charitable Foundation award. Tufts Graduate School of Biomedical Sciences
and Tufts University School of Medicine, Boston, MA
2014, Abstract selected as Best of American Heart Association Specialty Conferences: “T Cell Mediated
Immune Responses Regulate Cardiac Remodeling and Survival in Pressure Overload Induced
1. Goni O, Alcaide P and Fresno M. Immunosuppression during acute Trypanosoma cruzi infection: involvement of Ly6G(Gr1+)CD11b+ immature myeloid suppressor cells. Int Immunol, 2002, 14 (10): 1125- 1134.
2. Alcaide P and Fresno M. AgC10, a mucin from Trypanosoma cruzi, inhibits the proinflammatory activity of macrophages by inhibiting p38SAPK/MK2 and destabilizing TNF mRNA. Eur J Immunol, 2004, 34 (6):1695-704.
3. Alcaide P and Fresno M. AgC10, a membrane mucin from Trypanosoma cruzi, inhibits T cell activation leading to a decreased transcription of IL-2. Int Immunol. 2004, 16 (10):1365-75.
4. Kawakami A, Aikawa M, Alcaide P, Luscinskas FW, Libby P and Sacks FM. ApolipoproteinCIII in apoB lipoproteins enhances the adhesion of human monocytic cells to endothelial cells. Circulation, 2006,113
5. Harari OA, Alcaide P, Ahl D, Luscinskas FW and Liao JK. Absence of TRAM restricts Toll-like Receptor-4 signaling in vascular endothelial cells to the MyD88 pathway. Circ Res, 2006, 98 (9): 1134-40.
6. Xie C, Alcaide P, Song K, Schneider D, Herrmann M, Preissner KT, Luscinskas FW and Chavakis T. Suppression of experimental autoimmune encephalomyelitis by the anti-inflammatory extracellular adherence protein (Eap) of Staphylococcus aureus. J Exp Med, 2007, 204 (2): 431-9.
7. Cai YH, Alvarez A, Alcaide P, Duramad P, Lim YC, Jarolim P, Lowe JB, Luscinskas FW and Lichtman AH. Abrogation of functional selectin-ligand expression reduces migration of pathogenic CD8+ T cells into heart. J Immunol, 2006, 176(11):6568-75.
8. Kawakami A, Aikawa M, Alcaide P, Luscinskas FW, Libby P and Sacks FM. Apolipoprotein CIII induces expression of vascular cell adhesion molecule-1 in vascular endothelial cells and increases adhesion of monocytic cells. Circulation. 2006, 114(7): 681-7.
9. Swirski FK, Libby P, Aikawa E, Alcaide P, Luscinskas FW, Weissleder R and Pittet MJ. Ly-6Chi monocytes dominate hypercholesterolemia-associated monocytosis and give rise to macrophages in atheromata. J Clin Invest. 2007, 117(1): 195-205.
10. Lou O, Alcaide P, Luscinskas FW and Muller WA. CD99 is a key mediator of the transendothelial migration of neutrophils. J Immunol. 2007, 178(2): 1136-43.
11. Alcaide P, Jones TG, Lord GM, Glimcher LH, Hallgren J, Arinobu Y, Akashi K, Paterson AM, Gurish MA and Luscinskas FW. Dendritic cell expression of the transcription factor T-bet regulates mast cell progenitor homing to mucosal tissue. J Exp Med. 2007, 204 (2): 431-9.
12. Alcaide P, King SL, Dimitroff CJ, Lim YC, Fuhlbrigge RC and Luscinskas FW. The 130-kDa glycoform of CD43 functions as an E-Selectin ligand for activated Th1 Cells in vitro and in delayed-type hypersensitivity reactions in vivo. J Invest Dermatol. 2007, 127(8):1964-72.
13. Sircar M, Bradfield PF, Aurrand-Lions M, Fish RJ, Alcaide P, Yang L, Newton G, Lamont D, Sehrawat S, Mayadas T, Liang TW, Parkos CA, Imhof BA and Luscinskas FW. Neutrophil transmigration under shear flow conditions in vitro is junctional adhesion molecule-C independent. J Immunol. 2007; 178 (9):5879-87.
14. Rabodzey A, Alcaide P, Luscinskas FW and Ladoux B. Mechanical forces induced by the
transendothelial migration of human neutrophils. Biophys J. 2008; 95(3):1428-38. PMCID:
15. Noma K, Rikitake Y, Oyama N, Yan G, Alcaide P, Liu PY, Wang H, Ahl D, Sawada N, Okamoto R, Hiroi Y, Shimizu K, Luscinskas FW, Sun J and Liao JK. ROCK1 mediates leukocyte recruitment and neointima formation following vascular injury. J Clin Invest. 2008; 118(5):1632-44. PMCID: PMC2293333
16. Alcaide P, Auerbach S and Luscinskas FW. Neutrophil Recruitment Under Shear Flow: It's All About Endothelial Cell Rings And Gaps. Microcirculation. 2008; 16 (1): 43-57. PMCID: PMC2726622.
17. Alcaide P, Newton G, Sehrawat S, Mayadas-Norton T, Vincent PA, Yacono P, Golan DE, Kowalcyk A. and Luscinskas FW. p120-catenin regulates leukocyte transmigration through an effect on VE-Cadherin phosphorylation. Blood. 2008 Oct 1;112(7):2770-9. PMCID: PMC2556612.
18. Kasorn A, Alcaide P, Jia Y, Subramanian KK, Sarraj B, Li Y, Loison F, Hattori H, Silberstein LE, Luscinskas WF and Luo HR. Focal Adhesion Kinase Regulates Pathogen-Killing Capability and Life Span of Neutrophils via Mediating Both Adhesion-Dependent and -Independent Cellular Signals. J Immunol 2009, 183 (2):1032-43. PMID: 19561112
19. Jones TG, Hallgren J, Humbles A, Burwell T, Finkelman FD, Alcaide P, Austen KF and Gurish MF. Antigen-induced increases in pulmonary mast cell progenitor numbers depend on IL-9 and CD1drestricted NKT cells. J Immunol. 2009, 183(8):5251-60. PMCID: PMC2782612
20. Alcaide P, Lim YC, Luscinskas FW and Fresno M. Mucin AgC10 from Trypanosoma cruzi Interferes with L-Selectin Mediated Monocyte Adhesion. . Infect. Immun 2010, 78(3):1260-8. PMCID: PMC2825940.
21. Koduru S, Kumar L, Ozcan E, Oyoshi M, Massaad M, Lebron S, Ramesh N, Kaku M, Fujiwara Y, Kremer L, King S, Fuhlbrigge R, Sage P, Carman C, Alcaide P, Luscinskas FW and Geha RS. CIP4 is essential for integrin dependent T cell trafficking. Proc Natl Acad Sci U S A. 2010 107(37):16252-6. PMCID: PMC2941295
22. Zhang J, Alcaide P, Liu L, Sun J, He A, Luscinskas FW and Shi GP. Regulation of endothelial cell adhesion molecule expression by mast cells, macrophages, and neutrophils. PLoS One 2011;6(1):e14525. PMCID: PMC3021513
23. Williams MR, Azcutia V, Newton G, Alcaide P and Luscinskas FW. Emerging mechanisms of neutrophil recruitment across endothelium. Trends Immunol. 2011, 461-9. PMID: 21839681; PMCID: PMC3185121.
24. Maganto-García E, Bu D, Tarrio ML, Alcaide P, Newton G, Griffin GK, Croce KJ, Luscinskas FW, Lichtman AH and Grabie N. Foxp3+ Inducible Regulatory T cells Suppress Endothelial Activation and Leukocyte Recruitment. J Immunol. 2011, 187(7):3521-9.PMID: 21873519; PMCID: PMC3217244.
25. Alcaide P, Maganto-Garcia E, Newton G, Travers R, Croce KJ, Bu DX, Luscinskas FW and Lichtman AH. Difference in Th1 and Th17 lymphocyte adhesion to endothelium. J Immunol. 2012, 188(3):1421- 30. PMID: 22219321; PMCID: PMC3262911.
26. Griffin GK, Newton G, Tarrio ML, Bu DX, Maganto-Garcia E, Azcutia V, Alcaide P, Grabie N, Luscinskas FW, Croce KJ and Lichtman AH. IL-17 and TNF-α Sustain Neutrophil Recruitment during Inflammation through Synergistic Effects on Endothelial Activation. J Immunol. 2012, 188(12): 6287-99. PMID: 22566565; PMCID: PMC3370121.
27. Alcaide P, Martinelli R, Williams MR, Adam A, Vincent PA and Luscinskas FW. p120-catenin prevents neutrophil transmigration independently of Rho A inhibition by impairing Src dependent VE-cadherin phosphorylation. Am J Physiol Cell Physiol. 2012, 303(4):C385-95. PMCID: PMC3422989
28. Bene NC, Alcaide P, Wortis HH, Jaffe IZ. Mineralocorticoid receptors in immune cells: Emerging role in cardiovascular disease. Steroids, 2014; 91C:38-45. PMCID: PMC4205205
29. Massaad MJ, Oyoshi MK, Kane J, Koduru S, Alcaide P, Nakamura F, Ramesh N, Luscinskas FW, Hartwig J, Geha RS.Binding of WIP to Actin Is Essential for T Cell Actin Cytoskeleton Integrity and Tissue Homing. Mol Cell Biol. 2014; 34(23):4343-54. PMCID: PMC4248745.
30. Nevers T, Salvador AM, Grodecki-Pena A, Knapp A, Velazquez F, Aronovitz M, Kapur NK, Karas RH, Blanton RM and Alcaide P. Left ventricular T cell recruitment contributes to the pathogenesis of heart failure. In press, Circulation: Heart Failure.
Dr. Alcaide received her PhD in Molecular Biology from Universidad Autonoma of Madrid, Spain, where she studied the immunological aspects of Trypanosoma cruzi infection, the protozoan parasite that causes Chagas disease. As a recipient of a Fulbright postdoctoral fellowship, Dr. Alcaide trained in Dr. F.W Luscinskas laboratory in the Brigham and Women’s hospital where she trained in vascular biology and studied the mechanisms regulating immune cell trafficking. After completion of her postdoctoral research training, Dr. Alcaide was appointed to Instructor of Pathology at Harvard Medical School and successfully competed for an NIH K99/R00 award while in Dr. Luscinskas lab and later joined the faculty at MCRI in September 2011 to establish her independent research program.
The Alcaide lab is a vascular immunology research team that combines several immunology, vascular biology and cardiac physiology in vitro and in vivo approaches to study several aspects of recruitment of T lymphocytes in diverse inflammatory settings, with a particular focus in the heart in the context of heart failure. The over-arching goal in the lab is to better understand the processes that take place during T lymphocyte recruitment to the heart, both in the T lymphocytes and in the endothelial cells, and how those can potentially be targeted in therapeutically useful ways.
Why T lymphocytes? T cell recruitment into tissues is a hallmark of several chronic inflammatory processes. Among T cells, the subset of T helper type 17 (Th17) cells plays a major role in autoimmunity and chronic inflammation, and we found that its interactions with the vascular endothelium differ from other T cell subsets. The lab combines real time imaging videomicroscopy under flow conditions and two different mouse animal models of autoimmunity to study the specific mechanisms that regulate Th17 cell trafficking.
Why heart failure? Heart Failure (HF) is a leading cause of morbidity and mortality. This progressive syndrome is generally caused by a decline in left ventricular (LV) function that increases LV pressure and activates LV hypertrophy and fibrosis, a process known as cardiac remodeling. Chronic inflammation has been recently associated with the pathophysiology of HF. Our lab uses the mouse model of pressure overload induced HF to study the role of T cells and T cell subsets in HF, the mechanisms triggering T cell recruitment to the heart, and the ways infiltrated T cells impair cardiac function. In collaboration with cardiologists in the MCRI, our lab also studies the role of T cells in human non-ischemic HF.
The Alcaide lab is formed by scientists who are passionate about their research and have interests of applying their immunology and vascular biology knowledge to heart disease. Our lab emphasizes the importance of collaborative team work in a friendly and multicultural environment to achieve the lab’s main research goals.